- Zachary Quinto Spock
- Chris Pine Kirk
- Eric Bana Nero
- Zoe Saldaña Uhura
- Anton Yelchin Checkov
- Winona Ryder Amanda Grayson
- Simon Pegg Scotty
- Karl Urban Bones
- John Cho Sulu
- Leonard Nimoy Spock Prime
- Bruce Greenwood Pike
- Ben Cross Sarek
- Jennifer Morrison Winona Kirk
- Rachel Nichols Gaila
- Jimmy Bennett James T. Kirk (joven)
- Kelvin Yu Técnico médico
- Chris Hemsworth George Kirk
- Tim Griffin
- Scottie Thompson Mujer de Nero
- Pasha D. Lychnikoff Romulan Commander
